The Island is the third and last major area seen in Resident Evil 4. After defeating Ramon Salazar, Leon S. Kennedy gets a ride to the island where Ashley is held from Ada. Before being defiled by Saddler and Los Iluminados, the Island was what appeared to be a private military island off the coast of Spain. It featured several bases, including a top-secret research facility. It also had a hospital, a prison, a communications tower, and a ruins complex within its inner walls. The island is home to some of the most powerful and most fearsome enemies in the game.The Island is reached at Chapter 5-1 and continues until the Final Chapter. Common enemies here are Saddler's private Militia, armed with maces, shields, axes, crossbows, and stun rods. The sub-bosses include Gatling Man; the Regenerador and Iron Maiden. The bosses are U3, Jack Krauser and Osmund Saddler himself.
- During the Castle level, some zealots will say "No dejes que escapen a la isla vivo," which means "Do not let them escape to the island alive", revealing the next scenario in Leon's mission to rescue Ashley.
- The Island is the only area to appear in a single Chapter.