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Annette Birkin (アネット・バーキン Anetto bākin?) is a file that can be found in Resident Evil: The Darkside Chronicles.

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Annette is the mother of Sherry Birkin and the wife of the man responsible for the creation of the G-Virus, William Birkin. Annette gave birth to their daughter two years before William discovered the G-Virus. Even after becoming a mother, she threw herself back into her research out of love for her husband. Just before the biological disaster spread across the entire city, she contacted her daughter and instructed her to seek shelter at the police station. On her way to meet up with Sherry, she encountered Ada, the woman who was after her husband's G-Virus. Annette also suspected Leon of being a spy due to him protecting Ada, but when she finds out that the mutated William is searching for Sherry, she gives Leon information to help Sherry and resolves to make sure William is stopped.

G-ウィルス研究の第一人者であるウィリアム・バーキンの妻で、シェリーの母親。
ウィリアムが「G」を発見する2年前に娘のシェリーを出産。

母親となった後も、夫を愛するあまり研究に没頭したが、生物災害が街全体に広がる直前、アネットはシェリーに連絡をとり、警察署に避難するように指示した。

シェリーに合流する途中、アネットは夫婦の研究成果であるGを追うエイダに遭遇し、彼女を襲撃した。
その際、エイダを庇ったレオンにもスパイの疑いを抱くが、シェリーがウィリアムに追われている事を知ると、娘を助ける為の情報をレオンに伝え、夫を倒す決意を固める。

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