Upon entry, four tabs can be seen which requires stepping. Two statues can be push to each tabs while Leon and Ashley can be place at the tabs. This open the door to the Spiked Ceiling Trap room. A handgun ammo can be found at the right top corner of the room, inside the Spiked trap room. Ashley later will fell into a trap where a gate will sealed her and two Cultist driving a spiky vehicle attempt to attack her. Leon can save her by shooting at both Cultist where the gate will open. The Queen's Grail can be found on the chest.
You must shoot the four red glowing bulbs on the ceiling before the spikes impale you.
The insignia door before entering the room
(locked door sound) It won't open. "Four Knights shall clear the path"