Rookie police officer Leon drives into Raccoon City ready for his first day, soon discovering it besieged by Zombies. Joining fellow survivor Claire Redfield, the two drive in a police car to the Raccoon City Police Department in the hopes of finding help. Attacked by a Zombie from the back seat, they swerve 180 degrees and collide with the back of the station. A truck then crashes into the car, separating Leon and Claire on different ends of the road.
Leon makes his way into Kendo Gun Shop, where he meets the shopkeeper, who warns him that the entire town is infested before being killed. Leon enters the RPD through its front door and is introduced to his superior, Marvin Branagh, who warns him that the Zombie-related killings have been going on for months, but were ignored completely two months earlier after the S.T.A.R.S. team was discredited.
Ada gets the Club Key via the vent. Ben is implanted by Birkin and Chief Irons is killed by Birkin. Leon fights the G-creature and Claire confronts Birkin. After Ada chases her to the sewage maintenance area, Annette ambushes her and tells her about the G-Virus, the murder of her husband, and how the city had been infected. Annette tries to shoot her but Ada, with her quick and agile skills, manages to overthrow Annette, who falls down into the sewer. Leon learns from Ada that she was a spy. Ada tells Leon to hand her over the G-Virus, or she'll shoot him. However Leon doesn't know that there are no bullets in the gun's magazine. Annette is knocked out cold by a falling large pipe after a sudden, strong quake. The G-Virus sample from Sherry's pendant is tossed by Claire in the smelting pool. Annette however also has a sample and will be acquired by Leon. But Ada would force him to give her the sample. Ada is then shot from behind by Annette and Ada will fall down. Out of despair and grief, Leon threw the G-Virus sample away. He then must defeat the mutant Birkin in one of its final forms and escape by getting on the train.