Natalia fills the support role when accompanying Barry just like Moira with Claire. Natalia engages in battle by using bricks as her only mean of defense and to break locks and boxes when necessary. Additionally, she can either thrust the brick while holding it on downed enemies, or toss it at an enemy for a quick act of escape. Aside from bricks, Natalia is also able to utilize smokescreen bottles to momentarily blind enemies and briefly reveal Glasps.
Natalia has a special sensing ability that sets her apart from the other characters. When the player is controlling Natalia, a yellow colored aura can be seen around an enemy identifying they are nearby, including ones that are present in different rooms. Furthermore, the aura will change color to a dark red once the enemy is aware of Natalia or Barry's presence. (Note that these aura colors can be changed in the Options menu to the preference of the players.) Natalia is also able to use her unique ability to find insect larvae, which she can destroy with a brick. The player can use BP to upgrade the "Sixth Sense" skill, which will increase the range of Natalia's awareness.
When pressing the aim button, Natalia can point at an enemy to inform Barry that the "monster" is coming or is nearby. Natalia can also pinpoint a creature's weakness by pointing at it, as she can "see" a Revenant's core which must be shot to kill it. She can also use her unique point command to reveal hidden items in the darkness.
Since Natalia's physical appearance is much smaller than Barry's, she uses this advantage to pass through narrow areas; an example being when she crawls through a small hole and unlocks the door for Barry to pass. Another capability Natalia has is being able to unlock item boxes that Barry cannot open himself by using one of her small hands.
In the Extra Episode: Little Miss, Natalia is playable alongside Alex Wesker's copied consciousness in the form of Dark Natalia. The objective is to find the letters from Natalia's missing teddy bear, Lottie, and advance through each area without being caught. If Natalia is spotted by an enemy, she will crouch down in fear and proceed to "faint" as Dark Natalia taunts her, failing the mission as a result.
Natalia is unable to sense enemies, so she must rely on Dark Natalia's abilities in order to reach her destination. The only weapon available to her is the smokescreen bottle, which she can use to blind enemies and run past them without being detected. Also, Natalia is the only one who can pick up key items and open doors.