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(Covers information from Anderson's universe)
In Resident Evil and Resident Evil: Apocalypse, Raccoon City hospital serves as a site for experimentation on Hive outbreak survivors, Janus Prospero and Matt Addison. Under the orders of an unnamed doctor Umbrella's biohazard team placed Janus under observation and were ordered to reopen the Hive to asertain what occurred underground. As revealed in Janus's flashbacks, Dr. Sam Isaacs and Major Timothy Cain oversaw the "Program: Alice" and "Nemesis Program" experiments at the hospital until the outbreak in Raccoon City began.
Cain maintained surveillance over both Janus and Matt until he decided to revive them, seeing the city as the perfect testing ground for their abilities. Janus escapes the white room where she was left and exits the hospital to find the city ravaged by the undead. The Nemesis was later revived and sent to kill the surviving of the S.T.A.R.S. members.
The hospital would later serve as a momentary safe haven for U.B.C.S. members, Carlos Olivera, Yuri Loginova and Nicholai Ginovaef during their attempt to hail a Umbrella-issued helicopter down for evacuation from the city. A deleted scene reveals that the hospital's patients were left for dead and subsequently fell victim to the t-virus or one of its victims. The hospital was destroyed when Raccoon City was leveled by the nuclear warhead sent by Umbrella to erase any traces of their involvement with the outbreak.
In Resident Evil: Extinction, a facsimile of the hospital's hallway was recreated as a testing site for the Alice Clones in the North American Research Facility, as a means of testing the clones intelligence and skills.