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Claire Redfield, Leon Scott Kennedy, and Ingrid Hannigan reprise their roles from the video game series, and are voiced by the same actors.
Chris Redfield is referenced in the film when Leon and Claire discuss the career paths they took.
When Claire goes to look for survivors in the airport she was given an umbrella that had exactly the same colour pattern as Umbrella corporations logo to use as a weapon. Claire even noted the irony in the movie.
Soundtrack clips from Resident Evil 2 game are used in the theatrical trailer.
When Frederic Downing shuts his office door, the sound of it closing is the same sound of players closing doors in the early Resident Evil games.
At the end of the film, TRICELL is seen collecting the remains of Curtis. They would become one of the main antagonists in Resident Evil 5.
During Hardvardville's quarantine, there is a news reporter that has the same attire as Alyssa Ashcroft from the outbreak series.
This is the first film produced by Capcom. Hollywood's only contributions were the voice actors and live motion capture actors.
There is an iPhone game based on Degeneration.
Leon's jacket is modified slightly from his Bomber Jacket, to a leather HellCat Jacket.
In the Raccoon City flashback, while Leon talks with Claire, it shows Leon wielding his VP70 pistol and Claire wielding a M1100 Shotgun, which is odd, since Claire can't acquire a Shotgun in her storyline of Resident Evil 2.
In the movie the United States Marine Corps Soldiers are depicted wearing Army Combat Uniforms. The ACU's are strictly Army Issue, the Marine Corps have their own "Digital" camouflage pattern called MARPAT which is Woodland or Desert Colored.