The gameplay of Resident Evil Outbreak File 2 is basically the same as the first game which follows the original Resident Evil gameplay style tradition, but it includes a few other gameplay improvements.
It is now possible to move while firing.
You can now pick up items from the floor while crawling.
Alyssa and Kevin are able to potshot even in Danger status.
It is now possible to play multiples of the same "type" of character in an online game.
An apology ad-lib (voice command) has been added and is done by holding L2 and pressing Triangle.
A transferable extra starting item is included for all characters.
Alyssa now does a backwards jump instead of the back step.
Yoko cannot shoulder ram anymore and can only do the face push.
If attacked while holding someone, Cindy can make he or she duck.
Kevin can now do an elbow rush that knocks enemies back.
George has a stronger tackle that can knock enemies over.
Jim can swing a melee weapon very fast, but it leaves him winded afterward.
David has a fourth swing to his knife combo and two swings when aimed upward and downward.
Mark can push heavy objects and his big swing charges faster and can be aimed in all directions.
The game includes a new Nightmare Difficulty mode.